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The PCR used in library preparation for PGM was optimized on the basis of the different Tm values of the PGM fusion primers that indicate another variable to consider when comparing platforms. This indicates that there may be platform-specific influences on the identified abundances of certain lineages, which we confirmed by the BLASTp results obtained with the reference database. Summary sequencing statistics are given in Additional file 1: Table S2. DNA (2.0-10 μg in 200 μl 10 mM Tris–HCl pH8.5) was sheared in an AFA clear mini-tube using a Covaris S2 device (Covaris Inc.) with the following settings: Duty cycle 20, Intensity 0.1, cycles/burst 1000, 600 seconds. Поддерживает большинство популярных операционных систем, включая GNU/Linux, Microsoft Windows и Mac OS X. LibreOffice бесплатен и имеет открытый исходный код, следовательно, вы можете бесплатно скачивать, использовать и изучать LibreOffice.

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